Agricultural Engineering

Investigation of the tolerance to crude oil in the fungal strains isolated from the Maron oilfield of Ahvaz

Document Type : Research Paper

Authors

Farzaneh Dehdari 1
Mehdi Mehrabi-Koushki 2
Hamid Alvanipour 3
Jamshid Hayati 2

1 Former M. Sc. student, Department of Plant protection, Faculty of Agriculture, Shahid Chamran University of Ahvaz, Iran.

2 Associate Professor, Department of Plant protection, Faculty of Agriculture, Shahid Chamran University of Ahvaz, Iran.

3 Ph.D. in Mycology and Plant Pathology, Department of Plant protection, Faculty of Agriculture, Shahid Chamran University of Ahvaz, Iran.

https://doi.org/10.22055/agen.2024.46614.1720
Abstract
Introduction: Soil contamination by crude oil is common in oilfield in different processes including extraction, transfer and refining of crude oil and its products. Bioremediation is an interesting strategy to remediation of soils polluted with crude oils and its derivatives. This method is based on the employed of organisms to neutralize oils in the environment. During bioremediation process, microorganisms degrade and transform persistent crude oil hydrocarbons into simple and less persistent molecules. It is very important to identify the microorganisms that can utilize and degrade these pollutants. Generally, fungi play an essential role in the biodegrading and remediation of soils polluted with hydrocarbons. Therefore, the present study was carried out to evaluate the tolerance of the fungal isolates obtained from the oil-contaminated soil to crude oil in Maroon oilfield.
Materials and Methods: Twenty-three fungal isolates belonging to 12 genera were used to investigate their toleration to crude oil. The fungi had been isolated in December 2016 from soil samples contaminated with crude oils from four sites in the maroon oilfield of Ahwaz. The fungal isolates were obtained from the fungal collection of the department of plant protection of shahid chamran university of Ahvaz. The fungi had been identified based on phylogenetic analysis and morphological characteristics. The tolerance of these fungi to crude oil was studied by growing them on PDA medium containing 30, 40, and 50% concentrations of crude oil and through Radial growth measurements. The tolerance to crude oil was calculated based on growth inhibition percentage of fungal isolates. The research was conducted in a factorial completely randomized design for analysis of the growth-inhibitory percentages.Three replications were performed for each control (PDA-Tween culture medium without crude oil) and treatment. Mean data comparisons were performed based on Duncan's multi-range method at 1% significance level (P<0.01), using SAS 9.1 software.
Results and Discussion: All isolates grew in the culture medium containing different concentrations of crude oil. The results of variance analysis showed significant difference between the main effects of isolates and different levels of crude oil, as well as their interaction effects on growth inhibition (P< 0.01). The growth inhibition means of three concentrations of 50, 40, and 30% was 33.6, 26.1, and 21.4, respectively, which indicated the direct relationship between the concentration level and the growth inhibition percentage. Naturally crude oil is a heterogeneous mixture that composed of hydrocarbons compounds, sulfur and other heavy elements, therefore an increase in its concentration leads to an addition in its toxicity. Comparisons of the mean of growth inhibition in different isolates in response to crude oil concentrations demonstrated that Aspergillus sp. SCUA-Deh-3 with 65.1 inhibition growth and placed in the group a had the lowest growth ability and highest sensitivity to oil and statistically significant difference exists between this isolate and all fungi (P< 0.01). M. circinelloides SCUA-34 and Cladosporium puyae SCUA-m5f4 ranked next with 45.1 and 41.8 inhibition of growth respectively and were placed in group b. Alternaria destruens SCUA-Deh-1 and Aspergillus sp.SCUA with 5.05 and 6.5 inhibitions and placed in group M had the lowest sensitivity and inhibition and had significant difference with all other fungi (P> 0.01). Also, the growth rate of both used Alternaria isolates enhanced by increasing oil concentration in media. The growth inhibition means were equal only in two isolates, Penicillium chrysogenum SCUA-Deh-12 and Aspergillus sp. SCUA-m1f8r2, in two concentrations of 30 and 40 % (4.6 and 19.6, respectively). Also, Aspergillus sp. SCUA-m3f10 had equal means percent inhibition of growth in two concentrations of 40 and 50 (28.5). Furthermore, in the isolates of Aspergillus sp. SCUA-Deh-3 initially stimulated growth at 40% concentration and then the growth inhibition percentage increased at concentration of 50%.
Conclusion: These results showed that Alternaria destruens SCUA-Deh-1 and Aspergillus sp. SCUA-m1f7r2 have more growth potential than other fungi at presence of crude oil in growth medium. Also, the growth inhibition of Alternaria decreased by increasing of crude oil concentration. In other words, these two isolates were considered the most tolerant isolates to crude oil. So it seems; these native isolates are among the best fungi for bioremediation of oil-contaminated soils. However, regarding the biological degradation of petroleum materials, it seems necessary to mention that physicochemical properties and bioavailability of hydrocarbon pollutants play an important roles in their bioremediation.

Keywords

Growth inhibition
tolerance
Fungi
Maron
Crude oil
Subjects
References
1. Abu Al-Ghaith, S., and Zuait, A.A.M. 2020. Isolating, identifying, and testing the efficiency of some fungi in hydrocarbon decomposition from oil-contaminated soil. Journal of Applied Science, 4: 78-90.
2. Al-Hawash, A. B., Alkooranee, J. T., Abbood, H. A., Zhang, J., Sun, J., Zhang, X., and Ma, F. 2018). Isolation and characterization of two crude oil-degrading fungi strains from Rumaila oil field, Iraq. Biotechnology Reports, 17: 104-109.
3. Al-Jawhari, I.F.H., 2014. Ability of some soil fungi in biodegradation of petroleum hydrocarbon. Journal of Applied and Environmental Microbiology, 2 (2): 46-52.
4. Anonymous. 2024. https://www.opec.org/opec_web/en/
5. Asemoloye, M.D., Tosi, S., Daccò, C., Wang, X., Xu, S., Marchisio, M.A., Gao, W., Jonathan, S.G., and Pecoraro, L., 2020. Hydrocarbon degradation and enzyme activities of Aspergillus oryzae and Mucor irregularis isolated from nigerian crude oil-polluted sites. Microorganisms, 8 (12): 1912.
6. Daâssi, D., and Almaghrabi, F.Q. 2023. Petroleum-Degrading fungal isolates for the treatment of soil microcosms. Microorganisms, 11 (5): 1-20.
7. Dawoodi, V., Madani., M., and Tahmourespour, A. 2013. Flora of soil fungi in Khuzestan province's oil regions. Biological Journal of Microorganism, 3(10): 87-96. (in Persian).
8. Dehdari, V. 2018. Isolation and identification of oil tolerant fungi from lands affected by Marun oil field in Ahvaz. M.Sc. dissertation. Shahid Chamran University of Ahvaz. Khuzestan. (in Persian with English abstract).
9. El-Aziz, A. R. A., Al-Othman, M. R., Hisham, S. M., and Shehata, S. M. 2021. Evaluation of crude oil biodegradation using mixed fungal cultures. Plos One, 16 (8): 1-19.
10. Eshghi Malayeri, B., Mohsenzadeh, F., Chehregani, A., Shir Khani, Z., and Molaheydari, V. 2011. Study on the possibility of application of rhizospheral fungi for bioremediation of petroleum polluted soils. 14th National Congress On Environmental Health. Shahid Sadoughi University of Medical Sciences, Yazd. (in Persian with English abstract).
11. Fraga, M. E., Zonta, E., and Balieiro, F. C. 2011. Isolation and selection of filamentous fungi from petroleum contaminated soil. Bioresearch Bulletin, 4: 227-235.
12. Heidarytabar, Rezvan, Ehsan Azin., and Hamid Moghimi. 2017. Introduction of halotolerant Mucor circinelloides UTMC 5032 for bioremediation crude oil hydrocarbons Biological Journal of Microorganism, 6 (21): 31-45. (in Persian with English abstract (.
13. Koohkan, H., Golchin, A., Mortazavi, M. S., Hemati, R., and Shahryari, F. 2020. Reduction of crude oil pollution in soil by phytoremediation, bioaugmentation and bioaugmented phytoremediation. Iranian Journal of Soil and Water Research, 50 (10): 2645-2660.
14. Maamar, A., Lucchesi, M.E., Debaets, S., Nguyen van Long, N., Quemener, M., Coton, E., Bouderbala, M., Burgaud, G., and Matallah-Boutiba, A., 2020. Highlighting the crude oil bioremediation potential of marine fungi isolated from the port of Oran (Algeria). Diversity, 12 (5): 1-19.
15. Moghimi, H., Heidary Tabar, R., and Hamedi, J. 2017. Assessing the biodegradation of polycyclic aromatic hydrocarbons and laccase production by new fungus Trematophoma sp. UTMC 5003. World Journal of Microbiology and Biotechnology, 33: 1-10.
16. Mohammadian, Elham, Mahdi Arzanlou., and Asadollah Babai-Ahari. 2017 Diversity of culturable fungi inhabiting petroleum-contaminated soils in Southern Iran. Antonie Van Leeuwenhoek, 110: 903-923.
17. Mohsenzadeh, F., Nasseri, S., Mesdaghinia, A., Nabizadeh, R., Zafari, D., Khodakaramian, G., and Chehregani, A. 2010. Phytoremediation of petroleum-polluted soils: Application of Polygonum aviculare and its root-associated (penetrated) fungal strains for bioremediation of petroleum-polluted soils. Ecotoxicology and Environmental Safety, 73 (4): 613-619.
18. Mohsenzadeh, F., Chehregani Rad, A., and Akbari, M. 2012. Evaluation of oil removal efficiency and enzymatic activity in some fungal strains for bioremediation of petroleum-polluted soils. Iranian journal of environmental health science and engineering, 9: 1-8.
19. Mohsenzadeh F., Zafari D., and Noorisafa B. 2016. Adaptation of some fungal species of Trichoderma to petroleum pollution. Cellular and Molecular Research (Iranian Journal of Biology), 29 (3): 321-330. (in Persian with English abstract).
20. Neisi, A., Jorfi, S., Goudarzi, G., Ardakani, M.R., Mahmoudi, P., and Takdastan, A., 2024. Biodegradation potential of native hydrocarbon degrading bacteria by using bio-stimulation on crude oil in soils of Khuzestan province (Abadan, Ahvaz and Andimeshk)- Iran. Bioremediation Journal, 28 (1):79-88.
21. Obire, O., Aleruchi, O., and Wemedo, S. 2020. Fungi in biodegradation of polycyclic aromatic hydrocarbons in oilfield wastewater. Acta Scientific Microbiology, 3: 220-224.
22. Pourfakhraei, E., Badraghi1, J., Mamashli, F., Nazari, M., and Saboury, A.A. 2018. Biodegradation of asphaltene and petroleum compounds by a highly potent Daedaleopsis sp. Journal of Basic Microbiology, 58 (7): 609–622.
23. Romero-Hernández, L., Velez, P., Betanzo-Gutiérrez, I., Camacho-López, M. D., Vázquez-Duhalt, R., and Riquelme, M. 2021. Extra-heavy crude oil degradation by Alternaria sp. isolated from deep-sea sediments of the Gulf of Mexico. Applied Sciences, 11 (13): 1-15.
24. 24- Roshandel, F., Saadatmand, S., Iranbakhsh, A., and Oraghi, A.Z., 2021. Effect of petroleum contaminant on pin creating and amino acid content of Pleurotus florida (P. Kumm). Iranian Journal of Plant and Biotechnology, 16 (2): 19-30. (in Persian with English abstract).
25. Saravanan, A., Karishma, S., Kumar, P. S., and Rangasamy, G. 2023. Biodegradation of oil-contaminated aqueous ecosystem using an immobilized fungi biomass and kinetic study. Environmental Research, 220: 1-8.
26. Sari, E. M., Novianty, R., Awaluddin, A., and Pratiwi, N. W. 2019. Effectiveness of crude oil degrading fungi isolated from petroleum hydrocarbon contaminated soil in Siak, Riau. Acta Biochimica Indonesiana, 2 (1): 15-22.
27. Shafiq, S.A., and Mizil, S.N. 2009. Effect and utilIzation of crude oil on some of fungi isolated from soil. Ibn Al-Haitham Journal for Pure and Applied Sciences, 22: (4). (in Arabic with English abstract).
28. Sharifi Hosseini, S., Shahbazi, A., Yazdipour, A., and Kamranfa, I. 2009. The effect of agricultural fertilizers on bioremediation of a crude-oil polluted. Journal of Water and Soil, 23 (3): 14-155. (in Persian with English abstract).
29. Varjani, S. J. 2017. Microbial degradation of petroleum hydrocarbons. Bioresource Technology, 223: 277-286.
30. Wang, H., Kuang, S., Lang, Q., and Yu, W. 2018. Effects of aged oil sludge on soil physicochemical properties and fungal diversity revealed by high-throughput sequencing analysis, Archaea: 1-8.
31. Zafra, G., Absalón, Á.E., Cuevas, M.D.C., and Cortés-Espinosa, D.V. 2014. Isolation and selection of a highly tolerant microbial consortium with potential for PAH biodegradation from heavy crude oil-contaminated soils. Water, Air and Soil Pollution, 225: 1-18.
Agricultural Engineering
Volume 47, Issue 2
Spring 2024
Pages 185-200

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