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Effects of Micronutrients and Humic Substance Application on some Quantitative and Qualitative Traits of Medicinal and Aromatic Herbs of Istanbul oregano

Document Type : Research Paper

Authors

1 MSc Student, Department of Soil Science, Faculty of Agriculture, Urmia university

2 Department of soil science

3 Department of Agronomy

Abstract

Introduction The Lamiaceae is a chief medicinal and aromatic plant family. It is a family of extensive diversity and variety with international distribution. It is known that most of their properties are due to the essential oils they contain as products of their secondary metabolism. The metabolic performance of living organisms can be distinguished into primary and secondary metabolisms. Secondary metabolites represent features that can be expressed in terms of ecological, taxonomic, and biochemical differentiation and diversity. The antioxidant properties of the volatile oils are not as robust as that of water-soluble constituents. Oregano, a member of the family is an herb that has been cultivated for centuries in the Mediterranean region, although now it can be found in most continents. Because of variability in composition and origin of different Origanum spp, the antioxidant capacity reported can vary substantially. In one study, oregano had the highest total antioxidant capacity and phenolic content compared with other Lamiaceae herbs, thyme, sage, rosemary, mint, and sweet basil. Istanbul oregano (Origanum vulgare ssp. hirtum (Link) Ietswaart)is a perennial plant with dense indumentum, white and pink flowers, strong fragrance, and flowering in July and August. It grows widelyin the Thrace, Marmara, and west and southern regions of Turkey. The plant is richer in oil and carvacrol content than O. onites and if improved, agronomic affairs would offer a better alternative. The environmental and social reasons indicate that conventional agriculture constitutes a continuous pressure contributing to the progressive worsening of environmental conditions. In this matter, the increasing use of saline irrigation water is one of the main factors resulting in salt accumulation in the plant rhizosphere. In this situation, soil degradation emphasized the need to develop strategies of salt affected soil reclamation. One of the possible solutions is to use humic substances. Micronutrients are essential elements that are used by plants in small quantities. Yield and quality of agricultural products increased with micronutrients application; therefore, human and animal health is protected with a feed of enrichment plant materials. Each essential element only can perform its role in plant nutrition properly when other necessary elements are available in balanced ratios for the plant. The aim of this trial was to study the effects of some micronutrients and humic substances on the antioxidant activity of the plant.
Materials and Methods The trial was conducted at the experimental fields (37.53° N, 45.08° E, and 1320 m) and the greenhouse of the Agronomy Department, Faculty of Agriculture and the Lab of Biology Department, Urmia University, during 2015-2016, prepared in a randomized complete block design in three replications, using Fe, Zn, Cu, humic acid and their incorporation (3 g Lit-1) spray as treatments. The land was plowed at the optimum moisture level (field capacity) and leveled. Phosphorus and Potassium fertilizers were applied at pre-sowing time in autumn, according to soil analysis and farrowed in 50 cm. The seeds for sowing were obtained from Turkey. Sowing was done in green house at the Department of Horticulture, Faculty of Agriculture, Urmia University, during the period from 21. 03. 2015 till 06.05.2015. The seeds were sowed in plastic pots filled with soil, sand, and peat moss substrate as a material for germination. After that sowing was irrigated regularly depending on weather conditions and development stage of plants. Seedlings were harvested and planted in the experimental field. Nitrogen fertilizer was used in planting time, and vegetative phase according to soil analysis. Irrigation was conducted depending on plants need. Harvestings were done in 50% flowering. Physical and chemical characteristics of the field soil were determined by the standard method. Total phenolic content, flavonoid content, DPPH radical scavenging activity, nitric oxide radical scavenging activity, and Chain-breaking activitywere determined.
Results The aim of this study was to increase the amount of oregano essential oil, and the use of micronutrients and humic substances increased the essential oil content of the plant. According to the results, the highest and lowest leaf dry weight was related to Zn+Cu+HA+Fe and Cu treatments, respectively. The highest essential oil content (3.50%) was recorded in Zn+Cu+HA and the lowest (2.28%) in Fe+HA treatments. The highest amount of total phenol in the plant leaves (72.7 g gallic acid/ 100 g DW) in Zn, flavonoid content (88.8 g quercetin/ 100 g DW) in HA and DPPH radical scavenging activity (74.1%) were observed in Fe+Cu+Zn+HA sprayings. In addition maximum nitric oxide radical scavenging (50.3%) and Chain-breaking activity (70.7 -Abs-3/min/mg extract) were observed in Zn and Fe+Zn treatments, respectively. In conclusion, the type of micronutrients and humic substances can affect the quality traits such as essential oil and antioxidant activity and according to the use target of the plant, combined and non-combined consumption of micronutrients, and humic substances can be recommended.

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References
  1. Abedi, T, and Pakniyat, H. 2010. Antioxidant enzyme changes in response to drought stress in ten cultivars of oil seed rape (Brassica napus L.). Czech Journal of Genetics and Plant Breeding, 46(1): 27-34.
  2. Afraza, Z., Bolandi, M., Khorshidi, M. and Mohammadi Nafghi, A.A. 2014. Evaluation of antioxidant activity of aqueous and alcoholic extracts (Methanol, Ethanol) of petals of saffron. Journal of Agriculture and Technology Saffron, 3: 231-236.
  3. Alloway, B.J. 1995. Heavy Metals in Soils 2nd Ed. Blackie Academic and Professional, London.
  4. Almedia, L., Fernandes, E., Lima, J.C., Caste, P., Bahia, M.F. 2008. Walnut (Juglans regia) leaf extracts are strong scavengers of pro-oxidant reactive species. Food Chemistry, 106: 1014-1020.
  5. Alturkci, A., and Helal, M. 2004. Mobilization to Pb, Zn, Cu and Cd, in polluted soil. Soil Science Journal 7: 1972-1980.
  6. Amuamuha, L., Pirzad, A. and Hadi, H. 2012. Effect of varying concentrations and time of nanoiron foliar application on the yield and essential oil of pot marigold. International Research Journal of Applied and Basic Sciences, 3: 2085-209.
  7. Asekun, OT, Grieron, D.S, Afolayan, A.S. 2006. Influence of drying methods on the chemical composition and yield of the essential oil of Leonotis Leonurus. Journal of Scientific Research, 10: 61-64.
  8. Bang, B., Lee, H.S., Park, J.G., Choang, M.G., and Lim, J.D. 2007. Antioxidant activities and phenolic compound composition of extract from mulberry fruit. Journal of Medicinal Crop Science. 15(2): 120-127.
  9. Baydar, H., Sagdic, O., Ozkan, G., Karadogan, T. 2004. Antibacterial activity and composition of essential oils from Origanum, Thymbra and Satureja species with commercial importance in Turkey. Food Control: 15(3):169-72. 63
  10. Benkeblia, N. 2005. Free-Radical scavenging capacity and antioxidant properties of some selected onions and garlic extracts. Brazilian of Biology and Technology, 48(5): 753-759.
  11. Benton J., and Jones J.R. 2012. Plant Nutrition and Soil Fertility Manual, (2th Ed.), By Taylor and Francis Group, LLC, 351pp.
  12. Bergendi L, Benes L., Durackova Z, Ferencik M., 1999. Chemistry, physiology and pathology of free radical. Life Sciences Journal, 65:1865-1874.
  13. Bonilla J, Atares L, Chiralt, A., and Vargas, M., 2011. Recent patents on the use of antioxidant agents in food. Journal of Food, Nutrition and Agriculture, 3(2): 123-132.
  14. Bravo, L., 1998. Polyphenols: chemistry, dietary sources, metabolism, and nutritional significance. Nutrition Reviews, 56(11), pp.317-333.
  15. Burits, M., and Bucar, F., 2000. Antioxidant activity of Nigella sativa essential oil. Phytotherapy Research, 14(5), 323-328.
  16. Cakmak I., 2000. Possible roles of zinc in protecting plant cells from damage by reactive oxygen species. New Phytologist, 146:185–205.
  17. Chishti, S., Kaloo, Z.A. and Sultan, P. 2013. Medicinal importance of genus Origanum: A review. Journal of Pharmacognosy and Phytotherapy, 5(10):170-177.
  18. Cooper, R., Lavie, D., and Ctterman, Y., 1994. The distribution of rare phenolic type compound in wild and cultivated Wheats. Journal of Environments, 27: 331- 336.
  19. D’Antuono, L. F., Galletti, G. C., and Bocchini, P., 2000. Variability of essential oil content and composition of Origanum vulgare L. populations from a North Mediterranean area (Liguria region, Northern Italy). Annals of Botany, 86(3): 471-478.
  20. Dicko, H. M., Gruppen, H., Traore, A. S., Voragen, A. G. J. and Berkel, W. J. H. V. 2006. Phenolic compounds and related enzymes as determinants of Sorghum for food use. Biotechnology and Molecular Biology Review, 1(1): 21-38.
  21. Erdal, I., Kepenek, K., and Kizilgoz, I. 2004. Effect of foliar iron applications at different growth stages on iron and some nutrient concentrations in strawberry cultivars. Turkish Journal of Agriculture and Forestry, 28(6): 421-427.
  22. Gorinstein, S., Cvikrova, M., Machackova, I., Haruenkit, R., Zachwieja, Z., and Katrich, E., 2004. Characterization of antioxidant compounds in Jaffa sweeties and white grapefruits. Food Chemistry, 84: 503-510.
  23. Gulchin, I., Oktay, M., Krecci, E., Kufrevioglu, I. 2003. Screening of antioxidant and antimicrobial activities of anise (Pimpinella anisum L.) seed extracts. Food Chemistry, 83: 371-382.
  24. Hendawy, S., El-Sherbeny, S., Hossein, M., Khalid, Kh. and Ghazal, G. 2012. Response of two species of black cumin to foliar spray treatment. Australian Journal of Basic and Applied Sciences, 6: 636-642.
  25. Istrati, D., Vizireanu, C., Iordachescu, G., Dima, F. and Garnai, M., 2013. Physicochemical characteristics and antioxidant activity of goji fruits jam and jelly during storage. Annals of the University Dunarea de Jos of Galati Fascicle VI-Food Technology, 37(2).
  26. Jamshidi, M., Ahmadi Ashtiani, H. R., Rezazadeh, SH. A., Fathi, Azad, F., Mazandarani, M., and Khaki, A. 2010. Investigation and comparison of phenolic compounds of antioxidant activity of native chest native of Mazandaran. Quarterly Journal of Medicinal Plants, (2): 177-.183.
  27. Jia, Z., Tang, M., and Wu, J. 1999. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chemistry, 64: 555-559.
  28. Jordán, M.J., Martínez, R.M., Martínez, C., Monino, I., and Sotomayor, J.A., 2009. Polyphenolic extract and essential oil quality of Thymus zygis ssp. gracilis shrubs cultivated under different watering levels. Industrial Crops and Products, 29(1): 145-153.
  29. Karakurt, Y., Unlub, H., Unlub, H., and Pademb, H. 2009. The influence of foliar and soil fertilization of humic acid on yield and quality of pepper. Acta Agriculture Scandinavian, Section B, Soil and Plant Science, 59: 233-237.
  30. Kulisic, T., Radonic, A., Katalinic, V. and Milos, M. 2004. Use of different methods for testing antioxidative activity of oregano essential oil. Food Chemistry, 85(4): 633-640.
  31. Kumar, R., Sharma, S., Kaundal, M., Sharma, S. and Thakur, M., 2016. Response of damask rose (Rosa damascena Mill.) to foliar application of magnesium (Mg), copper (Cu) and zinc (Zn) sulphate under western Himalayas. Industrial Crops and Products, 596-602.
  32. Lagouri, V. and Boskou, D., 1996. Nutrient antioxidants in oregano. International journal of food sciences and nutrition, 47(6), pp: 493-497.
  33. Ling, T.Y., and Zhao, X.Y. 1995. The improved pyrogallol method by using terminating agent for superoxide dismutase measurement. Progress in Biochemistry and Biophysics, 22: 84-86.
  34. Malakouti, M.J., 2008. The effect of micronutrients in ensuring efficient use of macronutrients. Turkish Journal of Agriculture and Forestry, 32(3): 215-220.
  35. Marcocci, L., Packer, L., Droy-Lefai, MT., Sekaki, A. and Gardes-Albert, M. 1994. Antioxidant action of Ginkgo biloba extracts EGb 761. Methods of Enzymology, 234:462-475.
  36. Mehdi, S.S., Farzad, P., Hossein, H.D., Hamid, M., Majid, M., and Mohamad, R.T. 2013. Effect of intermittent furrow irrigation, humic acid and deficit irrigation on water use efficiency of sugar beet. Annals of Biological Research, 4 (3):187-193. (In Persian)
  37. Misra A, Dwivedi S, Srivastava A.K., Tewari D.K., Khan A and Kumar R., 2006. Low iron stress nutrition for evaluation of Fe- efficient genotype physiology, photosynthesis, and essential monoterpene oil yield of Ocmium sanctum. Photosynthetica, 44: 474 -77.
  38. Naczk, M., Wanasundara, P.K.J.P.D., and Shahidi, F. 1992. Facile spectrophotometric quantification method of sinapic acid in hexane-extracted and methanol-ammoniawater-treated mustard and rapeseed meals. Journal of Agricultural and Food Chemistry, 40(3)444-448.
  39. Nasiri, Y., Zehtab-Salmasi, S., Nasrullahzadeh, S., Najafi, S., Ghassemi-Golezani, K., 2010. Effects of foliar application of micronutrients (Fe and Zn) on flower yield and essential oil of chamomile (Matricaria chamomilla L.). Journal of Medicinal Plants Research, 4(17): 1733-1737.
  40. Nickavar, B., Mojab, F., and Dolat-Abadi, R. 2005. Analysis of the essential oils of two Thymus species from Iran. Food Chemistry, 90: 609-611.
  41. Nicoli, M.C., Calligaris, S., Mamzocco, L. 2000. Effect of enzymatic and chemical oxidation on the antioxidant capacity of catechin model systems and in apple derivatives. Journal of Agriculture and Food Chemistry, 48: 4576-4580.
  42. Oki, T., Masuda, M., Kobayashi, M., Nishiba, Y., Furuta, S., Suda, I., and Sat, T. 2002. Polymeric procyanidins as radical scavenging components in red-hulled rice. Journal of Agricultural and Food Chemistry, 50: 7525-7529.
  43. Peyvandi, M., Kamali-Jamakani, Z., and Mirza, M. 2012. The effect of nano-iron with iron chelate on the growth and activity of antioxidant enzymes in Satureja hortensis. New Cellular and Molecular Biotechnology Journal, 2 (5): 25 - 32.
  44. Rattanachaikunsopon, P., Phumkhachorn, P. 2010. Antimicrobial activity of basil (Ocimum basilicum) oil against Salmonella enteritidis in vitro and in food. Bioscience, Biotechnology, and Biochemistry, 74: 1200-1204.
  45. Ravi, S., Channal, H.T., Hebsur, N.S., Patil, B.N., and Dharmatti, P. 2008. Effect of sulphur, zinc and iron nutrition on growth, yield, nutrient uptake and quality of safflower (Carthamus tinctorius L.). Karnataka Journal Agriculture Science, 32: 382-385.
  46. Rice-Evans, C., Miller, N. and Paganga, G., 1997. Antioxidant properties of phenolic compounds. Trends in Plant Science, 2(4): 152-159.
  47. Rao, B.R. and Rajput, D.K. (2011). Response of palmarosa (Cymbopogon martinii (Roxb.) Wats. var. motia Burk.) to foliar application of magnesium and micronutrients. Industrial Crops and Products, 33(2): 277-281.
  48. Rion, B., and Alloway, J. 2004. Fundamental aspects of zinc in soils and plants. International Zinc Association. 23: 1-128.
  49. Said-Al Ahl, H.A.H. and Abeer A. Mahmoud, Effect of zinc and / or iron foliar application on growth and essential oil of sweet basil (Ocimum basilicum L.) under salt stress. Ozean Journal of Applied Sciences 3(1), 2010
  50. Said-Al Ahl, H.A.H., Omer, E.A., 2009. Effect of spraying with zinc and/or iron on growth and chemical composition of coriander (Coriandrum sativum L.) harvested at three stages of development. Journal of Medicinal Food Plant, 1 (2), 30–46.
  51. Salehi Surmaghi, M.H. 2010. Medicinal Plants and Phytotherapy. Nutrition world publications, Tehran, Iran, 3: 368-371.
  52. Shimoji, Y., Tamura, Y., Nakamura, Y., Nanda, K., Nishidai, S., Nishikawa, Y., Ishihara, N., Uenakai, K. and Ohigashi, H. 2002. Isolation and identification of DPPH radical scavenging compounds in Kurosu (Japanese unpolished rice vinegar). Journal of Agricultural and Food Chemistry, 50(22): 6501-6503.
  53. Sotiropoulou, D.E., and Karamanos, A.J. 2010. Field studies of nitrogen application on growth and yield of Greek oregano (Origanum vulgare ssp. hirtum (Link) Ietswaart). Industrial Crops and Products 32(3): 450-457.
  54. Stott, D.E. and Martin, J.P., 1990. Synthesis and degradation of natural and synthetic humic material in soils. Humic substances in soil and crop sciences, pp.37-63.
  55. Tan, K.H. 2003. Humic Matter in Soil and Environment. Marcel Dekker, New York, 408p.
  56. Tian, X., and Li, Y., 2006. Nitric oxide treatment alleviates drought stress in wheat seedlings. Plant Biology, 50: 775-778.
  57. Vinod, K., Awasthi, G., Chauchan, P.K. 2012. Cu and Zn tolerance and responses of the Biochemical and Physiochemical system of wheat. Journal of Stress Physiology and Biochemistry, 8: 3.
  58. Wink, D.A., Miranda, K.M., Espey, M.G., Pluta, R.M., Hewett, S.J., Colton, C., Vitek, M., Feelisch, M. and Grisham, M.B. 2001. Mechanisms of the antioxidant effects of nitric oxide. Antioxidants and Redox Signaling, 3(2): 203-213.
  59. Yadegari, M. 2015. Foliar application of micronutrients on essential oils of borago, thyme and marigold. Journal of Soil Science and Plant Nutrition, 15 (4): 949–964.
  60. Yanez-Mansilla, E., Cartes, P; Reyes-Diaz, M., Ribera-Fonseca, A., Rengel, Z., Alberdi, M. 2015. Leaf nitrogen thresholds ensuring high antioxidant features of Vaccinium corymbosum cultivars. Journal of Soil Science and Plant Nutrition, 15(3):574-586.
  61. Yung-Shin, S., Jau, T., Yuan-Tsung, C., Deng-Jue, Y. 2009. Evaluation of antioxidant ability of ethanolic extract from dill (Anethum graveolens L.) flower. Food Chemistry, 115(2): 515-521.
  62. Zhang, Z., Pang, X., Duan, X., Ji, Z. L. and Jiang, Y. 2005. Role of peroxidase in anthocyanine degradation in litchi fruit pericarp. Food Chemistry, 90: 47-52.
  63. Zhonggao, C., Felgines, O., Texier, C., Besson, D.J., Liu, J., Wang, S. 2005. Antioxidant activities of total pigment extract from blackberries. Food Technology and Biotechnology, 43 (1): 97-102.
Agricultural Engineering
Volume 42, Issue 2
June 2019
Pages 51-66
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